Role of Microbial Triose Phosphate Isomerase in Regulating Human Lung Cancer

The current work aims to study the role of Microbial TPI in regulating the Human Lung cancer. Studies showed that there was a relative increase in the activity of the TPI in the patients suffering with cancer. The protein showed an increased expression in colon and bladder carcinomas. The study was undertaken to un reveal the molecular basis of this relation and also to prove the same. The article involved the extraction of DNA from microbe lactobacillus and isolation and amplification of TPI gene using molecular techniques. Transformation of the gene into the compatible cells of the E Coli Dh5 alpha cells. The transformed cells are used as a source f TPI to act against the Human cancer Cell lines. The effect of these TPI cells on the cancer cell lines can be read using Colorimeter. The overall observation of the work was that there was a substantial effect of TPI gene on the cell line growth rate.